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	Provedor de dados BJMBR (2) Autor Abrantes,E.F. (1) Ferreira,L.R.P. (1) Gazzinelli,R.T. (1) Nascimento,F.R.F. (1) Pires,E.G. (1) Reis,L.F.L. (1) Ribeiro-Dias,F. (1) Russo,M. (1) Silva,A.M. (1) Mais... Palavra-chave TNF (2) Cytotoxicity (1) DDRT-PCR (1) Gene expression (1) Hydrogen peroxide (1) Inflammation (1) Interferon (1) L929 tumor cells (1) Macrophages (1) Nitric oxide (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Info:eu-repo/semantics/other (1) Ano 1999 (1) 1998 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Application of the differential display RT-PCR strategy for the identification of inflammation-related mouse genes BJMBR Silva,A.M.; Pires,E.G.; Abrantes,E.F.; Ferreira,L.R.P.; Gazzinelli,R.T.; Reis,L.F.L.. The inflammatory response elicited by various stimuli such as microbial products or cytokines is determined by differences in the pattern of cellular gene expression. We have used the differential display RT-PCR (DDRT-PCR) strategy to identify mRNAs that are differentially expressed in various murine cell types stimulated with pro-inflammatory cytokines, microbial products or anti-inflammatory drugs. Mouse embryonic fibroblasts (MEFs) were treated with IFNs, TNF, or sodium salicylate. Also, peritoneal macrophages from C3H/Hej mice were stimulated with T. cruzi-derived GPI-mucin and/or IFN-<FONT FACE="Symbol">g</FONT>. After DDRT-PCR, various cDNA fragments that were differentially represented on the sequencing gel were recovered, cloned and... Tipo: Info:eu-repo/semantics/article Palavras-chave: DDRT-PCR; Gene expression; Inflammation; TNF; Interferon. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700008 Cytotoxic activity of BCG-activated macrophages against L929 tumor cells is nitric oxide-dependent BJMBR Nascimento,F.R.F.; Ribeiro-Dias,F.; Russo,M.. The tumoricidal activity of activated macrophages has been attributed largely to the release of tumor necrosis factor (TNF), or to the production of reactive oxygen or nitrogen intermediates. The L929 tumor cell line (a murine fibroblast-like cell) when treated with actinomycin D (ActD) has been used to measure TNF<FONT FACE="Symbol">a</FONT> cytotoxicity. In the present study, we determined the cytotoxic activity of BCG-activated peritoneal macrophages against ActD-untreated L929 tumor cells. Furthermore, we measured the production of hydrogen peroxide (H2O2), nitric oxide (NO) and TNF by macrophages cultured in the presence or absence of L929 cells. As expected, BCG-activated macrophages produced significant amounts of H2O2 (16.0 ± 3.0 µM),... Tipo: Info:eu-repo/semantics/other Palavras-chave: Cytotoxicity; Macrophages; L929 tumor cells; Nitric oxide; TNF; Hydrogen peroxide. Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001200012 Registros recuperados: 2 Primeira ... 1 ... Última

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